Abstract

Magnetic hyperthermia therapy (MHT) has emerged as a promising non- invasive approach for tumor treatment. 磁気温熱療法（MHT）は、腫瘍治療における有望な非侵襲的治療法として注目されている。

This work presents a novel paradigm for the biomimetic synthesis of high-performance magnetoferritins and pave the way for their clinical application in MHT.

本研究は、高性能なマグネトフェリチンの生体模倣的合成に向けた新たなパラダイムを提示し、MHTにおけるその臨床応用への道を開くものである。

Introduction

語句の説明

MHT：Magnetic hyperthermia therapy

MNPs：magnetic nanoparticles

AMF：alternating magnetic field

safety limit H⋅f<5×109A⋅m−1⋅s−1H·f < 5 × 10^9 A·m^−1·s^−1

PNCs：magnetic protein nanocages

FOCs：ferroxidase centers

Dps：DNA-binding proteins

ferritins

virus capsid-like encapsulins

unique advantages: (1) The protein shell provides natural hydrophilicity and ensures excellent colloidal stability; (2) Their inherent biocompatibility minimizes inflammatory and immuno-logical responses [10]; (3) The protein architecture precisely controls the morphology, size, and crystallinity of the encapsulated magnetic core.

ferritin stands out as a ubiquitous iron-storage protein that plays a crucial role in cellular iron homeostasis

magnetoferritin:Magnetoferritin: in vitro synthesis of a novel magnetic protein. Science,

Owing to its exceptional biocompatibility, monodispersity, and chemical modifiability, magnetoferritin has found widespread applications in targeted drug delivery and magnetic resonance imaging (MRI) contrast agents

SAR: specific absorption rate

dual-strategy approach

Materials and methods

Materials

Ammonium ferrous sulfate:(NH4)2Fe(SO4)2⋅6H2O(NH_4)_2Fe(SO_4)_2 \cdot 6H_2O

cobalt(II) sulfate:CoSO4CoSO_4

Sodium chloride:NaCl

Tris

sodium hydroxide (NaOH)

methods

PcFn:Thermostable iron oxide nanoparticle synthesis within recombinant ferritins from the hyperthermophile Pyrococcus yayanosii CH1

anaerobic chamber

dosing system (TIAMO software)

The total metal ion concentration was maintained at 50 mmol·L−1 for all syntheses